熱帯植物のアスコルビン酸高集積機構の解明

内容の要約広島大学(Hiroshima University)博士(農学)Doctor of Agriculturedoctora

An intrathoracic scapular prolapse with hemorrhagic shock after a thoracotomy

We herein present a case in which an emergency operation was performed for an intrathoracic hemorrhage resulting from a scapular prolapse after a thoracotomy, a rare complication of this procedure. A 59-year-old man had undergone a right upper lobectomy with an extended resection of the posterior chest wall including the second to fourth ribs due to a direct invasion by a lung cancer. On postoperative day 80, we performed an emergency operation as the patient had gone into shock due to an intrathoracic hemorrhage with a right scapular prolapse. The scapula protruded through the enlarged fourth intercostal space. The prolapsed scapula was reduced and the defect in the chest wall was covered with Marlex mesh.ArticleINTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY. 12(2):326-327 (2011)journal articl

CDiP technology for reverse engineering of sporadic Alzheimer’s disease

Alzheimer’s disease (AD) is a neurodegenerative disease that causes cognitive impairment for which neither treatable nor preventable approaches have been confirmed. Although genetic factors are considered to contribute to sporadic AD, for the majority of AD patients, the exact causes of AD aren’t fully understood. For AD genetics, we developed cellular dissection of polygenicity (CDiP) technology to identify the smallest unit of AD, i.e., genetic factors at a cellular level. By CDiP, we found potential therapeutic targets, a rare variant for disease stratification, and polygenes to predict real-world AD by using the real-world data of AD cohort studies (Alzheimer’s Disease Neuroimaging Initiative: ADNI and Japanese Alzheimer’s Disease Neuroimaging Initiative: J-ADNI). In this review, we describe the components and results of CDiP in AD, induced pluripotent stem cell (iPSC) cohort, a cell genome-wide association study (cell GWAS), and machine learning. And finally, we discuss the future perspectives of CDiP technology for reverse engineering of sporadic AD toward AD eradication

Criterion of mechanical instabilities for dislocation structures

To understand the nature of mechanical instabilities of dislocation structures, which plays a central role, for example, in determining the plastic behavior and fatigue in crystalline metals, it is essential to investigate a critical condition in which a dislocation structure collapses. A criterion for the mechanical instability of arbitrary dislocation structures is proposed in this paper. According to the criterion, the mechanical instability can be described by the positiveness of the minimum eigenvalue of the Hessian matrix, which is composed by the second-order differential of potential energy of the system with respect to the dislocation coordinates. In addition, the collapse mode can be simultaneously determined by the eigenvector of the minimum eigenvalue. We applied the proposed criterion to the veins and dislocation walls under external loading, and it successfully describes the onset of instabilities and the corresponding collapse modes, regardless of the difference in structures and sizes. This success in the criterion paves the way to address the mechanical instability issues on more complex dislocation structures

Complete subsite mapping of a “loopful” GH19 chitinase from rye seeds based on its crystal structure

AbstractCrystallographic analysis of a mutated form of “loopful” GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)4 revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)4 molecules. One (GlcNAc)4 molecule bound to subsites −4 to −1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase

Functional disability trajectories at the end of life among Japanese older adults: findings from the Japan Gerontological Evaluation Study (JAGES)

BACKGROUND: this study aimed to identify distinct subgroups of trajectories of disability over time before 3 years of death and examine the factors associated with trajectory group membership probabilities among community-dwelling Japanese older adults aged 65 years and above. METHODS: participants included 4, 875 decedents from among community-dwelling Japanese older adults, aged ≥ 65 years at baseline (men: 3, 020; women: 1, 855). The certified long-term care levels of the national long-term care insurance (LTCI) system were used as an index of functional disability. We combined data from the 2010 Japan Gerontological Evaluation Study and data from the 2010 to 2016 LTCI system. Group-based mixture models and multinominal logistic regression models were used for data analysis. RESULTS: five distinct trajectories of functional disability in the last 3 years of life were identified: 'persistently severe disability' (10.3%), 'persistently mild disability' (13.0%), 'accelerated disability' (12.6%), 'catastrophic disability' (18.8%) and 'minimum disability' (45.2%). Multinominal logistic regression analysis found several factors associated with trajectory membership; self-rated health was a common predictor regardless of age and gender. The analysis also showed a paradoxical association; higher education was associated with trajectory group membership probabilities of more severe functional decline in men over 85 years at death. CONCLUSIONS: individual perception of health was a strong predictor of trajectories, independent of demographic factors and socio-economic status. Our findings contribute to the development of policies for the long-term care system, particularly for end-of-life care, in Asian countries

Spectral and temporal implementation of Japanese speakers' English vowel categories : a corpus-based study

This study investigates the predictions of second language (L2) speech acquisition models — SLM(-r), PAM(-L2), and L2LP — on how native (L1) Japanese speakers implement the spectral and temporal aspects of L2 American English vowel categories. Data were obtained from 102 L1 Japanese speakers in the J-AESOP corpus, which also includes nativelikeness judgments by trained phoneticians. Spectrally, speakers judged to be non-nativelike showed a strong influence from L1 categories, except L2 /ʌ/ which could be deflected away from L1 /a/ according to SLM(-r) and L2 /ɑː/ which seemed orthographically assimilated to L1 /o/ according to PAM(-L2). More nativelike speakers showed vowel spectra similar to those of native English speakers across all vowels, in accordance with L2LP. Temporally, although speakers tended to equate the phonetic length of English vowels with Japanese phonemic length distinctions, segment-level L1-L2 category similarity was not a significant predictor of the speakers’ nativelikeness. Instead, the implementation of prosodic-level factors such as stress and phrase-final lengthening were better predictors. The results highlight the importance of suprasegmental factors in successful category learning and also reveal a weakness in current models of L2 speech acquisition, which focus primarily on the segmental level. Theoretical and pedagogical implications are discussed

Construction of Reversible Lattice Molecular Automata

Several cellular automata (CA) models have been developed to simulate self-organization of multiple levels of structures. However, they do not obey microscopic reversibility and conservation laws. In this paper, we describe the construction of a reversible lattice molecular automata (RLMA) model, which simulates molecular interaction and self-organization of higher-order structures. The model's strict reversibility entails physically relevant conservation laws, and thus opens a way to precise application and validation of the methods from statistical physics in studying the necessary conditions for such multiple levels of self-organization.Comment: 29 pages, 20 figure

Impaired Differentiation of Osteoclasts in TREM-2–deficient Individuals

TREM-2 is an immunoglobulin-like cell surface receptor associated with DAP12/KARAP that activates monocyte-derived dendritic cells (DCs) in vitro. Recently, it has been shown that genetic defects of human DAP12/KARAP and TREM-2 result in a rare syndrome characterized by bone cysts and presenile dementia called Nasu-Hakola disease. This observation suggests that TREM-2 may function in myeloid cells other than DCs, most probably osteoclasts (OCs) and microglial cells, which are involved in bone modeling and brain function. Consistent with this prediction, here we show that OC differentiation is dramatically arrested in TREM-2–deficient patients, resulting in large aggregates of immature OCs that exhibit impaired bone resorptive activity. These results demonstrate a critical role for TREM-2 in the differentiation of mononuclear myeloid precursors into functional multinucleated OCs